| Product Name | Flag-Tag(HRP Conjugated) Mouse mAb |
| Cat Number | HR1AM2135 |
| Source | Mouse |
| Applications | WB |
| Species Reactivity | N/A |
| Recommended Dilutions | WB 1:5,000-10,000 |
| Specificity | N/A |
| Storage | PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles. |
| Clonality | Monoclonal |
| Isotope | IgG1 |
| Concentration | 1mg/ml |
| Observed Band | N/A |
| Gene ID | N/A |
| Alternative Name | DDDDK epitope tag, DYKDDDDK epitope tag, ECS epitope tag |
| Background | The DYKDDDDK peptide (Flag-tag) is a small component of an epitope which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. It has been used extensively as a general epitope tag in expression vectors. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.A Flag-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a Flag-tag to this protein allows one to follow the protein with an antibody against the Flag sequence. |
| Type | monoclonal |