Intrument | Microplate reader(405-414 nm) |
Assay Time | 25 min |
Validity | 6 |
Assay Type | Quantitative |
Sample Type | Serum,plasma,cell culture supernatant,tissue,cells |
Synonyms | GSH |
Detection Principal | Reduced GSH can react with Dinitrobenzoic acid (DNTB) to form a yellow complex which can be detected by colorimetric assay at 405 nm and calculate the reduced GSH content indirectly. |
Reagents | Normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4) |
Labware | Micropipettor, Vortex mixer |
Size | 96T |
Sensitivity | 2 ?mol/L |
Detection Range | 2-100 ?mol/L |
Recovery Rate | 96 |
Inter CV | 3.2 |