| Intrument | Microplate reader(520-550 nm,optimum wavelength: 540 nm) |
| Assay Time | 20 min |
| Validity | 6 |
| Assay Type | Quantitative |
| Sample Type | Serum,plasma |
| Synonyms | Mg |
| Detection Principal | The magnesium in the serum reacts with the complexometric indicator (Calmagite) to form the Calmagite-Mg compound. The absorbance of this compound at 540 nm is proportional to the concentration of magnesium in the sample. The concentration of magnesium can be calculated by measuring the OD value at 540 nm. |
| Reagents | Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4) |
| Labware | Micropipettor, Centrifuge, Incubator, Vortex mixer |
| Size | 96T |
| Sensitivity | 0.18 mmol/L |
| Detection Range | 0.18-2.50 mmol/L |
| Recovery Rate | 98 |
| Inter CV | 7.9 |