Intrument | Microplate reader(365-375 nm,optimum wavelength: 370 nm) |
Assay Time | 20 min |
Validity | 6 |
Assay Type | Quantitative |
Sample Type | Serum,plasma,tissue |
Detection Principal | Carbonyl can react with 2,4-dinitrophenylhydrazine and produce a kind of reddish brown hydrazone compounds, which has a specific absorbance peak at 370 nm. The content of carbonyl can be calculated according to the absorbance value. |
Reagents | Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4) |
Labware | Micropipettor, Centrifuge, Incubator, Vortex mixer |
Size | 96T |
Sensitivity | 1.29 ?g/mL |
Detection Range | 1.29-45 ?g/mL |
Recovery Rate | 101 |
Inter CV | 5 |