| Intrument | Microplate reader(565-595 nm,optimum wavelength: 580 nm) |
| Assay Time | 30 min |
| Validity | 6 |
| Assay Type | Quantitative |
| Sample Type | Serum,plasma,urine,saliva,milk |
| Synonyms | BUN |
| Detection Principal | Urea can be decomposed into ammonia ion and carbon dioxide by urease. Ammonia ion can react with amphyl and form a green substance in alkaline medium, and the production of the green substance is proportional to the urea content which can be calculated with the colorimetric assay at 580 nm. |
| Reagents | Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4) |
| Labware | Micropipettor, Centrifuge, Incubator, Vortex mixer |
| Size | 96T |
| Sensitivity | 0.09 mmol/L |
| Detection Range | 0.28-35 mmol/L |
| Recovery Rate | 104 |
| Inter CV | 4.3 |