| Intrument | Microplate reader(730-740 nm) |
| Assay Time | 20 min |
| Validity | 6 |
| Assay Type | Quantitative |
| Sample Type | Serum,plasma,urine,saliva,tissue,cells |
| Synonyms | T-AOC |
| Detection Principal | The principle of the ABTS method for determining the T-AOC is as follows. ABTS is oxidized to green ABTS+ by appropriate oxidant, which can be inhibited if there exist antioxidants. The T-AOC of the sample can be determined and calculated by measuring the absorbance of ABTS+ at 734 nm. Trolox is an analog of VE and has a similar antioxidant capacity to that of VE. Trolox is used as a reference for other antioxidant antioxidants. For example, the T-AOC of Trolox is 1, then the antioxidant capacity of the other substance with the same concentration is showed by the ratio of its antioxidant capacity to Trolox antioxidant capacity. |
| Reagents | Normal saline (0.9% NaCl), 80% Ethanol |
| Labware | Micropipettor, Centrifuge, Vortex mixer |
| Size | 96T |
| Sensitivity | 0.05 mmol/L |
| Detection Range | 0.05-1.00 mmol/L |
| Recovery Rate | 102 |
| Inter CV | 5 |