| Intrument | Spectrophotometer(405 nm) |
| Assay Time | 70 min |
| Validity | 6 |
| Assay Type | Enzyme Activity |
| Sample Type | Serum,plasma,cells,tissue |
| Synonyms | CAT |
| Detection Principal | The reaction that catalase (CAT) decomposes H2O2 can be quickly stopped by ammonium molybdate. The residual H2O2 reacts with ammonium molybdate to generate a yellowish complex. CAT activity can be calculated by production of the yellowish complex at 405 nm. |
| Reagents | Normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4) |
| Labware | Micropipettor, Vortex mixer, Incubator or Water-bath |
| Size | 100Assays |
| Sensitivity | 0.27 U/mL |
| Detection Range | 0.27-155.4 U/mL |
| Recovery Rate | 96 |
| Inter CV | 5.1 |