| Intrument | Microplate reader(530-540 nm,optimum wavelength: 536 nm) |
| Assay Time | 50min |
| Validity | 6 |
| Assay Type | Quantitative |
| Sample Type | Serum,plasma,tissue |
| Synonyms | VC,Ascorbic acid |
| Detection Principal | The most obvious chemical activity of VC is that reduce Fe3+ to Fe2+, then promote iron absorption in the intestine, promote the storage and utilization of iron. Fe3+ react immediately with reducing ascorbic acid to form Fe2+. then Fe2+ react with phenanthroline and the color developing reaction occurs. The content of vitamin C in sample can be determined. Measure the OD value and calculate the VC content indirectly. |
| Reagents | Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4), Absolute ethanol |
| Labware | Micropipettor, Centrifuge, Incubator, Vortex mixer |
| Size | 96T |
| Sensitivity | 0.31 ?g/mL |
| Detection Range | 0.31-20 ?g/mL |
| Recovery Rate | 108 |
| Inter CV | 6.1 |