Intrument | Spectrophotometer(340 nm) |
Assay Time | 60 min |
Validity | 3 |
Assay Type | Quantitative |
Sample Type | Serum,plasma,animal tissue,cells,cell culture supernatant |
Detection Principal | Glutamic acid can react with NAD+ under the catalysis of glutamate dehydrogenase to produce ?-ketoglutaric acid, NADH and NH4+. NADH has the maximum absorption at 340 nm. And glutamic acid content can be calculated by measuring the change of NADH. |
Reagents | Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4) |
Labware | Micropipettor, Centrifuge, Incubator, Water bath, Vortex mixer |
Size | 50Assays |
Sensitivity | 4.00 ?mol/L |
Detection Range | 4.00-450 ?mol/L |
Recovery Rate | 104 |
Inter CV | 2.9 |