| Intrument | Microplate reader(480-520 nm,optimum wavelength: 505 nm) |
| Assay Time | 30 min |
| Validity | 6 |
| Assay Type | Quantitative |
| Sample Type | Serum,plasma,tissue |
| Detection Principal | Pyruvic acid can react with chromogenic agent and the reaction product is reddish brown in alkaline solution. The depth of color is directly proportional to the pyruvate content. The pyruvate content can be calculated by measuring the OD value at 505 nm. |
| Reagents | Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4) |
| Labware | Micropipettor, Centrifuge, Incubator, Vortex mixer |
| Size | 96T |
| Sensitivity | 0.003 ?mol/mL |
| Detection Range | 0.003-2.0 ?mol/mL |
| Recovery Rate | 95 |
| Inter CV | 3.6 |