| Intrument | Microplate reader(510-530 nm,optimum wavelength: 520 nm) |
| Assay Time | 25 min |
| Validity | 6 |
| Assay Type | Quantitative |
| Sample Type | Serum,tissue |
| Synonyms | Fe |
| Detection Principal | Under the action of acidic solution and reductant, ferric ions can be separated from transferrin in serum, and reduced into ferrous ions (Fe2+). The latter then bind to bipyridine and form pink complexes. The concentration of iron can be calculated by measuring the OD value at 520 nm indirectly. |
| Reagents | Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4) |
| Labware | Micropipettor, Centrifuge, Water bath, Incubator, Vortex mixer |
| Size | 96T |
| Sensitivity | 0.08 mg/L |
| Detection Range | 0.29-10 mg/L |
| Recovery Rate | 96 |
| Inter CV | 2.8 |