Intrument | Microplate reader(600-620 nm,optimum wavelength: 600 nm) |
Assay Time | 30 min |
Validity | 3 |
Assay Type | Quantitative |
Sample Type | Serum,plasma,animal tissue,cells |
Synonyms | Cys |
Detection Principal | Phosphotungstic acid can be reduced by Cys and form tungsten blue, which has an absorption peak at 600 nm. Cys content can be calculated with the absorbance at 600 nm. |
Reagents | Phosphoric acid |
Labware | Micropipettor, Centrifuge, Water bath, Incubator, Vortex mixer |
Size | 96T |
Sensitivity | 0.03 mmol/L |
Detection Range | 0.07-2.0 mmol/L |
Recovery Rate | 94 |
Inter CV | 1.3 |