| Intrument | Microplate reader(660-670 nm,optimum wavelength: ?665 nm) |
| Assay Time | 80 min |
| Validity | 6 |
| Assay Type | Quantitative |
| Sample Type | Serum,plasma,animal tissue |
| Synonyms | H2S |
| Detection Principal | H2S can react with acetate solution to form ZnS which can be dissolved in alkaline solution. In the presence of Fe3+, methylene blue can be formed. Methylene blue has a maximum absorption peak at 665 nm. H2S content can be calculated indirectly by measuring the OD value at 665 nm. |
| Reagents | Normal saline (0.9% NaCl) |
| Labware | Micropipettor, Centrifuge, Incubator, Vortex mixer |
| Size | 96T |
| Sensitivity | 6.73 ?mol/L |
| Detection Range | 6.82-100 ?mol/L |
| Recovery Rate | 94 |
| Inter CV | 9.9 |