Intrument | Microplate reader(660-670 nm,optimum wavelength: ?665 nm) |
Assay Time | 80 min |
Validity | 6 |
Assay Type | Quantitative |
Sample Type | Serum,plasma,animal tissue |
Synonyms | H2S |
Detection Principal | H2S can react with acetate solution to form ZnS which can be dissolved in alkaline solution. In the presence of Fe3+, methylene blue can be formed. Methylene blue has a maximum absorption peak at 665 nm. H2S content can be calculated indirectly by measuring the OD value at 665 nm. |
Reagents | Normal saline (0.9% NaCl) |
Labware | Micropipettor, Centrifuge, Incubator, Vortex mixer |
Size | 96T |
Sensitivity | 6.73 ?mol/L |
Detection Range | 6.82-100 ?mol/L |
Recovery Rate | 94 |
Inter CV | 9.9 |