Intrument | Microplate reader(525-533 nm,optimum wavelength: 533 nm) |
Assay Time | 30 min |
Validity | 6 |
Assay Type | Quantitative |
Sample Type | Serum,plasma,tissue |
Synonyms | VE |
Detection Principal | Fe3+ can be deoxidized to Fe2+ by VE with ferroin existing. Fe2+ can react with phenanthroline and form pink compound under certain condition. After colorimetric assay, VE content can be figured out according to the standard curve or calculated through formula. |
Reagents | Normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4), Absolute ethanol, N-heptane |
Labware | Micropipettor, Centrifuge, Vortex mixer |
Size | 96T |
Sensitivity | 0.95 ?g/mL |
Detection Range | 0.95-40 ?g/mL |
Recovery Rate | 97 |
Inter CV | 6.3 |