| Intrument | Spectrophotometer(533 nm) |
| Assay Time | 60 min |
| Validity | 6 |
| Assay Type | Quantitative |
| Sample Type | Serum,plasma,tissue |
| Synonyms | VE |
| Detection Principal | Fe3+ can be deoxidized to Fe2+ by VE with ferroin existing. Fe2+ can react with phenanthroline and form pink compound under certain condition. VE content can be calculated by measuring the OD value at 532 nm. |
| Reagents | Normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4), Absolute ethanol,N-heptane |
| Labware | Micropipettor, Vortex mixer, Centrifuge |
| Size | 100Assays |
| Sensitivity | 0.09 ?g/mL |
| Detection Range | 0.09-40 ?g/mL |
| Recovery Rate | 98 |
| Inter CV | 4.9 |