| Intrument | Microplate reader(520-580 nm,optimum wavelength: 540 nm) |
| Assay Time | 20 min |
| Validity | 6 |
| Assay Type | Quantitative |
| Sample Type | Serum,plasma,tissue |
| Synonyms | TP |
| Detection Principal | Any compound that contains two -CONH2 in the molecule can react with alkaline copper solution to form a purple complex, which is known as the biuret reaction. Many peptide bonds (-CONH-) in protein molecules can perform this reaction, and the color degree of all kinds of proteins are essentially the same. |
| Reagents | Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4) |
| Labware | Micropipettor, Incubator, Vortex mixer, Centrifuge |
| Size | 96T |
| Sensitivity | 0.58 g/L |
| Detection Range | 0.58-100 g/L |
| Recovery Rate | 98 |
| Inter CV | 6.5 |