Intrument | Microplate reader(optimum wavelength: 412 nm) |
Assay Time | 20 min |
Validity | 6 |
Assay Type | Enzyme Activity |
Sample Type | Serum,plasma,animal tissue |
Synonyms | AchE |
Detection Principal | AchE catalyzes the hydrolysis of acetylcholine to form choline, and choline react with dithio p-nitrobenzoic acid (DTNB) to form 5-mercapto-nitrobenzoic acid (TNB). TNB has an absorption peak at 412nm. And the activity of AchE is calculated by measuring the increasing rate of absorbance at 412nm. |
Reagents | Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4) |
Labware | Micropipettor, Incubator, Vortex mixer, Centrifuge |
Size | 96T |
Sensitivity | 1.225 U/mL |
Detection Range | 1.225-490 U/mL |
Recovery Rate | 104 |
Inter CV | 9.3 |