| Intrument | Microplate reader(optimum wavelength: 412 nm) |
| Assay Time | 20 min |
| Validity | 6 |
| Assay Type | Enzyme Activity |
| Sample Type | Serum,plasma,animal tissue |
| Synonyms | AchE |
| Detection Principal | AchE catalyzes the hydrolysis of acetylcholine to form choline, and choline react with dithio p-nitrobenzoic acid (DTNB) to form 5-mercapto-nitrobenzoic acid (TNB). TNB has an absorption peak at 412nm. And the activity of AchE is calculated by measuring the increasing rate of absorbance at 412nm. |
| Reagents | Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4) |
| Labware | Micropipettor, Incubator, Vortex mixer, Centrifuge |
| Size | 96T |
| Sensitivity | 1.225 U/mL |
| Detection Range | 1.225-490 U/mL |
| Recovery Rate | 104 |
| Inter CV | 9.3 |