| Intrument | Microplate reader(620 nm-690 nm,optimum wavelength: 660 nm) |
| Assay Time | 40 min |
| Validity | 6 |
| Assay Type | Quantitative |
| Sample Type | Serum,plasma,tissue |
| Synonyms | Pi |
| Detection Principal | Inorganic phosphorus react with molybdic acid to produce phosphomolybdic acid. Phosphomolybdic acid can be reduced to molybdenum blue under the action of reducing agent. And the molybdenum blue have a maximum absorption peak at 660 nm. The phosphorus content can be calculated indirectly be measuring the OD value at 660 nm. |
| Reagents | Normal saline (0.9% NaCl) |
| Labware | Micropipettor, Incubator, Vortex mixer, Centrifuge |
| Size | 96T |
| Sensitivity | 0.004 mmol/L |
| Detection Range | 0.004-2.0 mmol/L |
| Recovery Rate | 101 |
| Inter CV | 3 |