Intrument | Spectrophotometer(545 nm) |
Assay Time | 60 min |
Validity | 3 |
Assay Type | Quantitative |
Sample Type | Tissue,mitochondria,other liquid samples |
Synonyms | CA |
Detection Principal | In acidic condition, Cr (?) will be reduced to Cr3+, Cr3+ reacts with citric acid. And the product has a characteristic absorption peak at 545 nm, therefore the content of citric acid in sample can be calculated by measuring the absorbance value at 545 nm. |
Reagents | Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4) |
Labware | Micropipettor, Water bath, Incubator, Vortex mixer, Centrifuge |
Size | 100Assays |
Sensitivity | 0.05 mmol/L |
Detection Range | 0.05-5.0 mmol/L |
Recovery Rate | 96 |
Inter CV | 5.4 |