| Intrument | Spectrophotometer(545 nm) |
| Assay Time | 60 min |
| Validity | 3 |
| Assay Type | Quantitative |
| Sample Type | Tissue,mitochondria,other liquid samples |
| Synonyms | CA |
| Detection Principal | In acidic condition, Cr (?) will be reduced to Cr3+, Cr3+ reacts with citric acid. And the product has a characteristic absorption peak at 545 nm, therefore the content of citric acid in sample can be calculated by measuring the absorbance value at 545 nm. |
| Reagents | Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4) |
| Labware | Micropipettor, Water bath, Incubator, Vortex mixer, Centrifuge |
| Size | 100Assays |
| Sensitivity | 0.05 mmol/L |
| Detection Range | 0.05-5.0 mmol/L |
| Recovery Rate | 96 |
| Inter CV | 5.4 |