| Intrument | Microplate reader(440-460 nm,optimum wavelength: 450 nm) |
| Assay Time | 35 min |
| Validity | 6 |
| Assay Type | Activity |
| Sample Type | Serum,plasma,urine,cells,cell culture supernatant,leucocyte |
| Detection Principal | Superoxide anion free radicals are produced through the reaction system of xanthine and xanthine oxidase. WST-1 (a water-soluble tetrazolium salt) can react with the generated superoxide anion to produce water-soluble formazan. When the tested sample contains the superoxide anion free radical inhibitor, it can inhibit the formation of formazan. When the tested sample contains the substance that produces superoxide anion free radical, it can promote the formation of formazan dye. By colorimetric analysis of WST-1 products, the units of activity of inhibition or production of superoxide anion radical in samples can be calculated. |
| Reagents | Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4) |
| Labware | Micropipettor, Multi-channel pipettor, Incubator, Vortex mixer, Centrifuge |
| Size | 96T |
| Recovery Rate | 100 |
| Inter CV | 5.8 |