| Intrument | Microplate reader (510 nm) |
| Assay Time | 30 min |
| Validity | 6 |
| Assay Type | Quantitative |
| Sample Type | serum, plasma, cells and tissue |
| Synonyms | TG |
| Detection Principal | Triglycerides (TG) can be hydrolyzed by lipoprotein lipase into glycerol and free fatty acids. Glycerol produces glycerol-3-phosphate and ADP under the catalysis of glycerol kinase (GK). Glycerol-3-phosphate produces hydrogen peroxide under the action of glycerol phosphate oxidase (GPO). In the presence of 4-aminoantipyrine and phenol, hydrogen peroxide is catalyzed by peroxidase (POD) to produce quinones which is proportional to the content of TG. |
| Reagents | Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4), Isopropanol(AR) |
| Labware | Micropipettor, Water bath, Incubator, Vortex mixer, Centrifuge |
| Size | 96T |
| Sensitivity | 0.14 mmol/L |
| Detection Range | 0.14-10 mmol/L |
| Recovery Rate | 105 |
| Inter CV | 9.2 |