Intrument | Microplate reader(540 nm) |
Assay Time | 40 min |
Validity | 6 |
Assay Type | Enzyme Activity |
Sample Type | animal and plant tissue |
Detection Principal | The reducing sugar reacts with 3,5-dinitrosalicylic acid under heating conditions to produce a brown-red substance, which is inactivated by the thermolabile nature of ?-amylase, and then the enzyme activity of ?-amylase is determined. |
Labware | Micropipettor, Multi-channel pipettor, Incubator, Vortex mixer, Centrifuge |
Size | 96T |
Sensitivity | 0.97 U/g tissue |
Detection Range | 0.97-34.74 U/g tissu |
Recovery Rate | 98 |
Inter CV | 3.9 |