Vitamin C (VC) Colorimetric Assay Kit

Vitamin C (VC) Colorimetric Assay Kit

Cat No.:HR3BC1122
Product available

  Product info

Detection Method: Colorimetric method

Storage : 2-8?
Product Details

Intrument Spectrophotometer(533 nm)
Assay Time 80 min
Assay TypeQuantitative
Sample TypeSerum,plasma,tissue
Synonyms VC,Ascorbic acid
Detection Principal The most obvious chemical activity of VC is that reduce Fe3+ to Fe2+, then promote iron absorption in the intestine, promote the storage and utilization of iron. Fe3+ react immediately with reducing ascorbic acid to form Fe2+. then Fe2+ react with phenanthroline and the color developing reaction occurs. The content of vitamin C in sample can be determined. Measure the OD value and calculate the VC content indirectly.
ReagentsNormal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4), Absolute ethanol
LabwareMicropipettor, Vortex mixer, Incubator, Centrifuge
Sensitivity 0.35 ?g/mL
Detection Range 0.35-20 ?g/mL
Recovery Rate 104
Inter CV 3.3

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