Intrument | Fluorescence microplate reader (Ex/Em=535 nm/587 nm) |
Assay Time | 20 min |
Validity | 6 |
Assay Type | Enzyme Activity |
Sample Type | Serum, plasma, animal tissue |
Synonyms | MPO |
Detection Principal | Under the catalysis of peroxidase, hydrogen peroxide can oxidize the non-fluorescent probe into the fluorescent substance, and its fluorescence intensity is proportional to the total peroxidase activity in the sample. This kit specifically inhibits the peroxidase activity of MPO in the sample through an MPO enzyme inhibitor, thus distinguishing the peroxidase activity of MPO in the sample from that of other peroxidases. |
Labware | Micropipette, Vortex mixer, Water bath |
Size | 96T |
Sensitivity | 0.001 U/L |
Detection Range | 0.001 - 1.26 U/L |
Inter CV | 5.4 |