Intrument | Fluorescence microplate reader (Ex/Em=535 nm/587 nm) |
Assay Time | 40 min |
Validity | 6 |
Assay Type | Quantitative |
Sample Type | Serum, plasma, urine, animal tissue |
Synonyms | UA |
Detection Principal | Uricase catalyzes the decomposition of uric acid into allantoin, CO2 and H2O2. Under the action of peroxidase, H2O2 oxidizes the non-fluorescent probe into the fluorescent substance. By measuring the fluorescence value of the system, the corresponding uric acid content can be calculated. |
Labware | Micropipettor, Vortex mixer, Centrifuge |
Size | 96T |
Sensitivity | 0.03 ?mol/L |
Detection Range | 0.03-15 ?mol/L |
Recovery Rate | 101 |
Inter CV | 7.2 |