| Intrument | Fluorescence microplate reader (Ex/Em=535 nm/590 nm) |
| Assay Time | 20 min |
| Validity | 6 |
| Assay Type | Quantitative |
| Sample Type | Serum, plasma, animal tissue, cells |
| Detection Principal | Total Cholesterol (TC) includes free cholesterol (FC) and cholesteryl esters (CE). Cholesterol ester can be hydrolyzed by cholesterol esterase to produce cholesterol and free fatty acid. Cholesterol is oxidized by cholesterol oxidase to produce ?4-cholestenone and hydrogen peroxide. In the presence of the enzyme and probe, hydrogen peroxide can be catalyzed to produce the fluorescence substrate. The fluorescence intensity at the excitation wavelength of 535 nm and emission wavelength of 590 nm is proportional to the cholesterol concentration. |
| Labware | Micropipettor, Vortex mixer, Centrifuge |
| Size | 96T |
| Sensitivity | 0.12 ?mol/L |
| Detection Range | 0.12-30 ?mol/L |
| Recovery Rate | 96 |
| Inter CV | 7.3 |