| Intrument | Fluorescence microplate reader (Ex/Em=535 nm/590 nm) |
| Assay Time | 25 min |
| Validity | 6 |
| Assay Type | Quantitative |
| Sample Type | Serum, plasma, urine |
| Synonyms | GLU |
| Detection Principal | Glucose oxidase can catalyze the oxidation of glucose into gluconic acid and produce hydrogen peroxide. In the presence of peroxidase, hydrogen peroxide reacts with the non-fluorescent substance to form fluorescent substance. The glucose content can be calculated indirectly by measuring the fluorescence intensity at the excitation wavelength of 535 nm and the emission wavelength of 590 nm. |
| Labware | Pipettor, Water bath, Centrifuge |
| Size | 96T |
| Sensitivity | 0.1 ?mol/L |
| Detection Range | 0.1-20 ?mol/L |
| Recovery Rate | 99 |
| Inter CV | 2.8 |