| Intrument | Fluorescence microplate reader (Ex/Em=535 nm/587 nm) |
| Assay Time | 75 min |
| Validity | 6 |
| Assay Type | Enzyme Activity |
| Sample Type | Serum, plasma, animal tissue |
| Synonyms | ALT |
| Detection Principal | ALT catalyze the amino conversion reaction between alanine and ?-ketoglutaric acid to produce pyruvic acid and glutamic acid. Under the action of pyruvate oxidase, pyruvic acid generates H2O2, which reacts with the non-fluorescent substance to form fluorescent substance under the action of peroxidase. The activity of ALT can be calculated by measuring the increase of fluorescence value at the excitation wavelength of 535 nm and the emission wavelength of 590 nm. |
| Labware | Pipettor, Vortex mixer, Centrifuge |
| Size | 96T |
| Sensitivity | 0.01 U/L |
| Detection Range | 0.01-0.83 U/L |
| Inter CV | 9.8 |