| Intrument | Microplate reader(520 nm-550 nm,optimum wavelength: 530 nm) |
| Assay Time | 20 min |
| Validity | 6 |
| Assay Type | Quantitative |
| Sample Type | Serum, plasma, animal tissue |
| Synonyms | D-LA |
| Detection Principal | Using NAD+ as H+ receptor, D-lactate dehydrogenase (LDH) catalyzes the reaction of D-lactic acid and NAD+ to generate pyruvic acid and NADH respectively. NBT is reduced to a kind of purple compound during the reaction. Measure the OD value at 530 nm, and the concentration of D-lactic acid can be calculated.#N/A |
| Reagents | PBS (0.01 M, pH 7.4) |
| Labware | Micropipettor, Vortex mixer, Incubator, Centrifuge |
| Size | 96T |
| Sensitivity | 0.06 mmol/L |
| Detection Range | 0.06-8.0 mmol/L |
| Recovery Rate | 99 |
| Inter CV | 7.7 |