| Intrument | Microplate reader(500 nm-520 nm,optimum wavelength: 505 nm) |
| Assay Time | 60 min |
| Validity | 6 |
| Assay Type | Enzyme Activity |
| Sample Type | Animal tissue |
| Detection Principal | Maltase catalyze the corresponding substrate to produce monosaccharide. Monosaccharide produce hydrogen peroxide under the action of oxidase. Hydrogen peroxide react with chromogenic agent to form red product. The activity of maltase can be calculated by detection the optical density with spectrophotometer at 505 nm. |
| Reagents | Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4) |
| Labware | Micropipettor, Vortex mixer, Centrifuge, Water bath, Incubator |
| Size | 96T |
| Sensitivity | 6.32 U/mL |
| Detection Range | 6.32-750 U/mL |
| Recovery Rate | 103 |
| Inter CV | 5.7 |