Intrument | Microplate reader(450 nm-470 nm,optimum wavelength: 460 nm) |
Assay Time | 65 min |
Validity | 6 |
Assay Type | Enzyme Activity |
Sample Type | Serum, plasma, animal tissue |
Synonyms | MPO |
Detection Principal | Myeloperoxidase reduces hydrogen peroxide to a complex. The complex react with o-dianisidine (as hydrogen donor) to produce a yellow product which has a maximum absorption peak at 460 nm. The activity of MPO can be calculated indirectly by measuring the OD value at 460nm. |
Labware | Tubes, Micropipette, Vortex mixer, 37?/60? Water bath |
Size | 96T |
Sensitivity | 19.42 U/L |
Detection Range | 19.42-893.31 U/L |
Recovery Rate | 104 |
Inter CV | 7.3 |