| Intrument | Microplate reader(324 nm) |
| Assay Time | 65 min |
| Validity | 3 |
| Assay Type | Enzyme Activity |
| Sample Type | Animal tissue |
| Synonyms | ChAT |
| Detection Principal | Acetyl-CoA can react with choline under the catalysis of choline acetyltransferase (ChAT) to produce coenzyme A (CoA), CoA can combine with the 4, 4-dithiopyridine. The activity of ChAT can be calculated indirectly by measuring the OD value at 324 nm. |
| Reagents | Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4) |
| Labware | Test tube, Centrifuge, 100? Water bath, Incubator. |
| Size | 96T |
| Sensitivity | 1.21 U/g fresh weigh |
| Detection Range | 1.21-40 U/g fresh we |
| Inter CV | 9.4 |