Intrument | Microplate reader(405 nm) |
Assay Time | 20 min |
Validity | 3 |
Assay Type | Enzyme Activity |
Sample Type | Serum, plasma, animal tissue |
Synonyms | GGT/?-GT |
Detection Principal | ?-GT catalyzes the transfer of gamma glutamyl group from glutamyl p-nitroaniline to N-glycyl glycine to produce p-nitroaniline, which has characteristic absorption peak at 405nm. The activity of ?-GT can be calculated according to the changing rate of absorbance value. |
Labware | Micropipettor, Vortex mixer, Centrifuge, Water bath, Incubator |
Size | 96T |
Sensitivity | 0.88 U/L |
Detection Range | 0.88-399.4 U/L |
Inter CV | 6.2 |