| Intrument | Microplate reader(495 nm-510 nm,optimum wavelength: 505 nm) |
| Assay Time | 60 min |
| Validity | 6 |
| Assay Type | Enzyme Activity |
| Sample Type | Animal tissue |
| Detection Principal | Lactase decomposes lactose to produce glucose. Under the action of enzyme, glucose produces hydrogen peroxide. In the presence of chromogenic oxygen receptors, peroxidase catalyzes hydrogen peroxide to produce colored substances. Lactase activity can be calculated by measuring the OD value at 505 nm. |
| Reagents | Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4) |
| Labware | Micropipettor, Vortex mixer, Centrifuge, Water bath, Incubator |
| Size | 96T |
| Sensitivity | 3.94 U/mL |
| Detection Range | 12.5-2000 U/mL. |
| Recovery Rate | 102 |
| Inter CV | 8.5 |