| Intrument | Spectrophotometer(505 nm) |
| Assay Time | 100 min |
| Validity | 6 |
| Assay Type | Enzyme Activity |
| Sample Type | Serum, plasma, animal tissue |
| Synonyms | AST,GOT |
| Detection Principal | AST enables alpha-ketoglutaric acid and aspartic acid to displace amino to form glutamic acid and oxaloacetic acid. Oxaloacetic acid can decarboxylate itself to form Pyroracemic acid during the reaction. Pyroracemic acid reacted with 2,4-dinitrophenylhydrazine (DNPH) to form 2,4-dinitrophenylhydrazone showing reddish brown in alkaline solution. Measure the OD values and calculate the enzyme activity. |
| Reagents | Normal saline (0.9% NaCl) |
| Labware | Test tube, Micropipettor, 37? Water bath, Vortex mixer |
| Size | 100 assays |
| Sensitivity | 0.38 IU/L |
| Detection Range | 0.38-72.30 IU/L |
| Recovery Rate | 104 |
| Inter CV | 4.9 |