| Intrument | Microplate reader(410 nm-420 nm,optimum wavelength: 412 nm) |
| Assay Time | 20 min |
| Validity | 6 |
| Assay Type | Quantitative |
| Sample Type | Serum, plasma, animal tissue |
| Synonyms | T-SH |
| Detection Principal | Sulfhydryl compounds react with 5,5' -dithiobis (2-nitrobenzoic acid) under neutral or alkaline conditions to produce a yellow product which have a maximum absorption peak at 412 nm. Measure the OD value and calculate the total mercapto content indirectly. |
| Reagents | Absolute ethyl alcohol |
| Labware | Micropipettor, Water bath, Incubator, Vortex mixer, Centrifuge |
| Size | 96T |
| Sensitivity | 9.91 ?mol/L |
| Detection Range | 9.91-1000 ?mol/L |
| Recovery Rate | 104 |
| Inter CV | 2.9 |