| Intrument | Microplate reader(440-460 nm,optimum wavelength: 450 nm) |
| Assay Time | 45 min |
| Validity | 6 |
| Assay Type | Enzyme Activity |
| Sample Type | Serum,plasma,hydrothorax,tissue,cells |
| Synonyms | LDH |
| Detection Principal | Using coenzyme I as a hydrogen carrier, LDH catalyze lactic acid to produce pyruvate. Pyruvate reacted with 2, 4-dinitrophenylhydrazine to form pyruvate dinitrophenylhydrazone, which was red-brown in alkaline solution, and the color depth was proportional to pyruvate concentration. The activity of LDH could be calculated by measuring OD value. |
| Reagents | Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4) |
| Labware | Micropipettor, Multichannel pipette, Incubator |
| Size | 96T |
| Sensitivity | 6 U/L |
| Detection Range | 6-1000 U/L |
| Recovery Rate | 98 |
| Inter CV | 2.4 |