Lactate Dehydrogenase (LDH) Activity Assay Kit

Lactate Dehydrogenase (LDH) Activity Assay Kit

Cat No.:HR3BC1125
Product available

  Product info

Detection Method: Colorimetric method

Storage : 2-8?
Product Details

Intrument Microplate reader(440-460 nm,optimum wavelength: 450 nm)
Assay Time 45 min
Assay TypeEnzyme Activity
Sample TypeSerum,plasma,hydrothorax,tissue,cells
Synonyms LDH
Detection Principal Using coenzyme I as a hydrogen carrier, LDH catalyze lactic acid to produce pyruvate. Pyruvate reacted with 2, 4-dinitrophenylhydrazine to form pyruvate dinitrophenylhydrazone, which was red-brown in alkaline solution, and the color depth was proportional to pyruvate concentration. The activity of LDH could be calculated by measuring OD value.
ReagentsNormal saline (0.9% NaCl), PBS (0.01 M, pH 7.4)
LabwareMicropipettor, Multichannel pipette, Incubator
Sensitivity 6 U/L
Detection Range 6-1000 U/L
Recovery Rate 98
Inter CV 2.4

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