Intrument | Spectrophotometer(290 nm) |
Assay Time | 70 min |
Validity | 6 |
Assay Type | Enzyme Activity |
Sample Type | Serum, plasma, animal tissue, cells |
Synonyms | PAL |
Detection Principal | Phenylalanine ammonia lyase (PAL) can catalyze L-phenylalanine to produce trans-cinnamic acid and ammonia, and trans-cinnamic acid has the maximum absorption peak at 290 nm. PAL activity can be calculated by measuring the increase of OD value at 290 nm. |
Labware | Tubes, Micropipettor, Vortex mixer, 37?C Incubator |
Size | 100 assays |
Sensitivity | 0.78 U/g tissue |
Detection Range | 0.78-156 U/g tissue |
Recovery Rate | 99 |
Inter CV | 4.6 |