Intrument | Microplate reader(640 nm-680 nm,optimum wavelength: 660 nm) |
Assay Time | 35 min |
Validity | 6 |
Assay Type | Enzyme Activity |
Sample Type | Serum, plasma, animal tissue |
Synonyms | Na+K+-ATPase |
Detection Principal | Na+K+-ATPase decomposes ATP to produce ADP and Phosphorus, and calculates the activity of Na+K+-ATPase by measuring the content of phosphorus. |
Reagents | Normal saline (0.9% NaCl) |
Labware | Micropipettor, Tubes, Vortex mixer, Incubator, 37? Water bath, Centrifuge. |
Size | 96T |
Sensitivity | 0.11 ?mol Pi/mL/hour |
Detection Range | 0.42-4.99 ?mol Pi/mL |
Recovery Rate | 104 |
Inter CV | 2.2 |