| Intrument | Microplate reader(405 nm) |
| Assay Time | 25 min |
| Validity | 6 |
| Assay Type | Enzyme Activity |
| Sample Type | Serum, plasma, animal tissue |
| Synonyms | LAP |
| Detection Principal | LAP can catalyze the substrate L-leucine-4-nitroaniline to produce p-nitroaniline, which has the maximum absorption peak at the wavelength of 405 nm. The enzyme activity of LAP can be calculated by measuring the increasing OD value of the system. |
| Reagents | Acetone |
| Labware | Micropipettor, Water bath, Centrifuge, Incubator, Vortex mixer |
| Size | 96T |
| Sensitivity | 5.2 U/L |
| Detection Range | 5.2-201.8 U/L |
| Inter CV | 6.4 |