| Intrument | Microplate reader(650 nm-670 nm,optimum wavelength: 660 nm) |
| Assay Time | 30 min |
| Validity | 6 |
| Assay Type | Quantitative |
| Sample Type | serum, plasma, urine, cellular supernatant, animal, plant tissue |
| Synonyms | TAS |
| Detection Principal | ABTS is oxidized to green ABTS?+ by appropriate oxidant, which can be reduced to colorless ABTS in the presence of antioxidants. The TAS of the sample can be determined and calculated by measuring the absorbance of ABTS?+ at 660 nm. Trolox is an analog of VE and has a similar antioxidant state to that of VE. Trolox is used as a reference substance for total antioxidant status. |
| Reagents | 60% Ethanol |
| Labware | Micropipettor, 37? incubator. |
| Size | 96T |
| Sensitivity | 0.23 mmol Trolox Equiv./L |
| Detection Range | 0.23-2 mmol Trolox Equiv./L |
| Recovery Rate | 99 |
| Inter CV | 7 |