| Intrument | Microplate reader(580 nm-590 nm,optimum wavelength: 590 nm) |
| Assay Time | 30 min |
| Validity | 3 |
| Assay Type | Quantitative |
| Sample Type | tissue, serum and other liquid samples |
| Synonyms | TOS |
| Detection Principal | Under acid conditions, the oxidizing material in the sample can oxidize Fe2+ to Fe3+, which binds highly with xylenol orange to produce a blue-purple complex. When the pH of solution is in the range of 2-3, its maximum absorption wavelength is around 590 nm, and the color depth is proportional to the content of oxidation substances in a certain concentration and a certain time, so as to indirectly calculate the total oxidation state of the sample. |
| Reagents | Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4) |
| Labware | Micropipettor, 37? incubator. |
| Size | 96T |
| Sensitivity | 2.5 ?mol H2O2 Equiv./L |
| Detection Range | 2.5-100 ?mol H2O2 Equiv./L |
| Inter CV | 3.5 |