| Intrument | Spectrophotometer (450 nm) |
| Assay Time | 70 min |
| Validity | 6 |
| Assay Type | Enzyme Activity |
| Sample Type | Serum, plasma, tissue, cells |
| Synonyms | LDH |
| Detection Principal | Lactate dehydrogenase (LDH) is an oxidoreductase. LDH catalyzes the conversion of lactate to pyruvic acid and back, as it converts NAD+ to NADH and back. LDH is composed of four subunits (tetramer). The two most common subunits are the LDH-M and LDH-H protein. LDH is released into the blood by cells after tissue damage or erythrocyte hemolysis. Extracellular LDH activity is used to detect cell damage or cell death. |
| Reagents | Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4) |
| Labware | Micropipettor, Vortex mixer, Water bath |
| Size | 100 assays |
| Sensitivity | 4 U/L |
| Detection Range | 4-400 U/L |
| Inter CV | 7.2 |