| Intrument | Microplate reader (340 nm) |
| Assay Time | 60 min |
| Validity | 3 |
| Assay Type | Quantitative |
| Sample Type | Serum, plasma, animal tissue, cells, cell culture supernatant |
| Detection Principal | Glutamate is a dicarboxylic acid, the most abundant amino acid in the cell, which can be converted into aminobutyric acid (GABA), ornithine, ketoglutarate, glucose or glutathione. Glutamate links carbohydrate and amino acid metabolism through the tricarboxylic acid (TCA) cycle. In the liver, it can regulate the rate of `ammonia to urea. In the central nervous system, it can act as an excitatory neurotransmitter. |
| Reagents | PBS (0.01 M, pH 7.4) |
| Labware | Micropipettor, 37? Water bath, Centrifuge, Incubator |
| Size | 96T |
| Sensitivity | 6.43 ?mol/L |
| Detection Range | 6.43-407 ?mol/L |
| Recovery Rate | 98 |
| Inter CV | 8.3 |