| Intrument | Spectrophotometer(520 nm) |
| Assay Time | 60 min |
| Validity | 6 |
| Assay Type | Enzyme Activity |
| Sample Type | Whole blood,serum,plasma,tissue,cells |
| Synonyms | CHE |
| Detection Principal | Cholinesterase breaks down acetylcholine into choline and acetic acid. Acetylcholine that is not hydrolyzed by cholinesterase reacts with basic hydroxylamine to form acetamidamine. It reacts in an acidic solution to form a brown-red hydroxamate iron complex. The color depth is directly proportional to the amount of remaining acetylcholine, which can be colorimetrically quantified. Cholinesterase activity was calculated. |
| Reagents | Normal saline (0.9% NaCl) |
| Labware | Micropipettor, Centrifuge, Incubator, Water bath, Vortex mixer |
| Size | 50Assays |
| Sensitivity | 1.17 U/mL |
| Detection Range | 1.17-160 U/mL |
| Recovery Rate | 104 |
| Inter CV | 9.4 |