| Intrument | Microplate reader(410 nm-430 nm,optimum wavelength: 420 nm) |
| Assay Time | 60 min |
| Validity | 6 |
| Assay Type | Enzyme Activity |
| Sample Type | Plant tissue |
| Synonyms | POD |
| Detection Principal | Plant peroxidase, a member of the superfamily of peroxidase, catalyzes the redox reaction between H2O2 and various reductants. The plant peroxidase has the same general structure and consists of iron porphyrin IX and ten ?-helixes. Based on the difference of primary structure, the superfamily of plant peroxidase can be divided into three types: class I (intracellular type), class II (extracellular type of fungi) and class III (secreted type of plant). |
| Reagents | PBS (0.01 M, pH 7.4) |
| Labware | Spectrophotometer (240 nm), Micropipettor, Vortex mixer, Centrifuge, 37? Incubator |
| Size | 96T |
| Sensitivity | 0.01 U/mL |
| Detection Range | 0.01?100 U/mL |
| Recovery Rate | 102 |
| Inter CV | 5 |