Intrument | Microplate reader (450 nm) |
Assay Time | 30 min |
Validity | 6 |
Assay Type | Enzyme Activity |
Sample Type | Serum, plasma, animal tissue, hydrothorax, cells |
Synonyms | LDH |
Detection Principal | Lactate dehydrogenase (LDH) is an oxidoreductase. LDH catalyzes the conversion of lactate to pyruvic acid and back, as it converts NAD+ to NADH and back. LDH is composed of four subunits (tetramer). The two most common subunits are the LDH-M and LDH-H protein. LDH is released into the blood by cells after tissue damage or erythrocyte hemolysis. Extracellular LDH activity is used to detect cell damage or cell death |
Labware | Microplate, Micropipettor, Vortex mixer, Water bath |
Size | 96T |
Sensitivity | 0.11 U/L |
Detection Range | 0.11-39.9 U/L |
Inter CV | 2.3 |