| Intrument | Fluorescence microplate reader (Ex/Em=535 nm/587 nm) |
| Assay Time | 50min |
| Validity | 6 |
| Assay Type | Quantitative |
| Sample Type | Animal liver and muscle tissue |
| Detection Principal | Glycogen produces glucose under the action of starch glycosidase, and glucose is catalyzed by glucose oxidase to produce hydrogen peroxide. In the presence of the peroxidase, hydrogen peroxide be oxidized to produce the fluorescence substrate. The fluorescence intensity at the excitation wavelength of 535 nm and emission wavelength of 587 nm is proportional to the glycogen content. |
| Labware | Micropipettor, Incubator, Centrifuge |
| Size | 96T |
| Sensitivity | 0.06 ?g/mL |
| Detection Range | 0.06-4.0 ?g/mL |
| Inter CV | 6.6 |