Intrument | Spectrophotometer(628 nm) |
Assay Time | 50 min |
Validity | 6 |
Assay Type | Quantitative |
Sample Type | Serum,plasma |
Synonyms | ALB |
Detection Principal | Bromocresol green (BCG) can combine with the albumin in pH 4.0~4.2 to form an albumin-BCG complex, which is yellowish-green. The depth of yellowish-green is proportional to the concentration of albumin. The serum albumin concentration can be calculated by measuring the OD value at 628 nm. |
Reagents | Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4) |
Labware | Micropipettor, Vortex mixer |
Size | 100Assays |
Sensitivity | 0.50 g/L |
Detection Range | 0.50-70 g/L |
Recovery Rate | 99 |
Inter CV | 4.2 |