| Intrument | Fluorescence microplate reader (Ex/Em=530 nm/590 nm) |
| Assay Time | 100 min |
| Validity | 6 |
| Assay Type | Quantitative |
| Sample Type | cells |
| Detection Principal | 2-DG is up-taken by the cells, converted to 2-DG-6P, which is catalyzed by glucose dehydrogenase to produce 6PDG. Meanwhile, NADP+ is converted to NADPH. The generated NADPH converts the probe into fluorescent substances under the action of myocardial yellow transferase. The glucose uptake can be calculated by measuring the fluorescence intensity at the excitation wavelength of 530 nm and the emission wavelength of 590 nm. |
| Reagents | KRPH solution (20 mM Hepes, 5 mM KH2PO4, 1 mM MgSO4, 1 mM CaCl2, 136 mM NaCl, 4.7 mM KCl, 2% BSA, pH 7.4) |
| Labware | Micropipettor, Incubator, Water bath |
| Size | 96T |
| Sensitivity | 0.02 nmol/?L |
| Detection Range | 0.02-0.5 nmol/?L |